The OVUCHECK^® PLASMA test is based on the competitive binding of unlabeled progesterone present in the standard or sample, and a fixed quantity of progesterone labelled with the enzyme alkaline phosphatase (AP) (conjugate), to binding sites on a limited amount of specific progesterone antibodies. The wells are pre-coated with antibodies, providing a solid phase for the capture of the progesterone present in the samples, the standards or the conjugate. After incubation, all components other than those bound to the plate wells are washed away. The amount of bound AP-labelled progesterone remaining on the wells is inversely proportional to the concentration of the unlabeled progesterone present in the sample. The bound labelled progesterone is then measured by making the AP react with its substrate during a second incubation. The colour produced is measured spectrophotometrically and the concentration of progesterone in the sample is determined from a standard curve.

• Qualitative test
• Competitive binding of progesterone
• Standard curve determined by progesterone standards (1, 2.5, 5 and 10 ng/mL)
  Tests per kit: 92 possible tests