Specific antibodies produced against pathogens responsible of digestive diseases have been immobilized on alternate rows of 8 x 12 wells-microtiter plates. These antibodies allow specifically the capture of the corresponding pathogens which are present in the feaces samples. Rows A, C, E and G have been sensitized with these antibodies and rows B, D, F and H are containing nonspecific antibodies. These rows allow the differentiation between specific immunological reaction and nonspecific bindings to get ride of false positives. Feaces are diluted in dilution buffer and incubated on the plate for one hour at room temperature. After this first incubation step, the plate is washed, then conjugates-peroxidase labeled anti-pathogen monoclonal antibodies are added to the wells and these HRPO conjugates will get linked to the antigens. The formation of an enzyme-labeled immunocomplexes detected by the appearance of a blue colour, when the substrate TMB is added. The intensity of the resulting blue colour is proportionate to the content of pathogenic elements in the sample. Acidification of the wells with stop solution stops the reaction and engenders a shift of the colour from blue to yellow that allows reading of the optical density at 450 nm. The signals recorded for the negative control microwells are subtracted from the corresponding positive microwells. The positive antigens supplied in the kit give the reference values.